3ALX
Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (MV-H(L482R)-SLAM(N102H/R108Y) fusion)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.713, 170.074, 110.681 |
Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
Resolution | 19.970 - 3.150 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zb6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.138 | 0.496 |
Number of reflections | 52457 | |
<I/σ(I)> | 10.3 | 2.9 |
Completeness [%] | 99.5 | 98.8 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion, floating drop | 8.3 | 293 | 0.1M imidazole pH8.3, 0.5M (NH4)2SO4, 0.7M Li2SO4, 0.05M Li(AcO)2, 1% ethylene glycol, vapor diffusion, floating drop, temperature 293K |