3AL3
Crystal Structure of TopBP1 BRCT7/8-BACH1 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-01 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 78.069, 78.069, 136.766 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.900 - 2.150 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3al2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.258 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.060 | 0.026 | 0.526 |
Number of reflections | 14063 | ||
<I/σ(I)> | 28.987 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 6.8 | 6.3 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Sodium formate, pH 8, vapor diffusion, hanging drop, temperature 298K |