3AKO
Crystal Structure of the Reassembled Venus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.054, 116.046, 156.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 2.100 |
| R-factor | 0.17007 |
| Rwork | 0.167 |
| R-free | 0.22919 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1myw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.780 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.102 | 0.329 |
| Number of reflections | 60532 | |
| <I/σ(I)> | 23.9 | 5.8 |
| Completeness [%] | 94.7 | 99.9 |
| Redundancy | 11 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 3350, 0.1M HEPES, 0.2M lithium sulfate , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
