3AKJ

Crystal structure of A Helicobacter pylori proinflammatory kinase CtkA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	255.195, 56.980, 48.638
Unit cell angles	90.00, 93.28, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.1962
Rwork	0.194
R-free	0.22970
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Number of reflections	47319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	295	100mM Tris-HCl, 1.5M AMS, 15% glycerol, pH 8.5, VAPOR DIFFUSION, temperature 295K
1	VAPOR DIFFUSION	8.5	295	100mM Tris-HCl, 1.5M AMS, 15% glycerol, pH 8.5, VAPOR DIFFUSION, temperature 295K