3AJW
Structure of FliJ, a soluble component of flagellar type III export apparatus
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.925, 52.925, 192.597 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.300 - 2.100 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.520 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.317 |
| Number of reflections | 10032 | |
| <I/σ(I)> | 25 | 7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.8 | 293 | 27% PEG 300, 0.1M Phospho-Citrate, 0.35M NaCl, pH 3.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
