3AJM

Crystal structure of programmed cell death 10 in complex with inositol 1,3,4,5-tetrakisphosphate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-10-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97888, 0.97928, 0.96405
Spacegroup name	P 41 21 2
Unit cell lengths	89.830, 89.830, 114.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.915 - 2.300
R-factor	0.216
Rwork	0.213
R-free	0.26410
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.676
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.083	0.317
Number of reflections	21428
<I/σ(I)>	14.6	5.8
Completeness [%]	99.9	100
Redundancy	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K