3AIH
Human OS-9 MRH domain complexed with alpha3,alpha6-Man5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.100, 73.100, 180.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.469 |
| Number of reflections | 17480 | |
| <I/σ(I)> | 28.5 | 5.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 11.4 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.9M ammonium sulfate, 0.1M Tris-HCl, 2% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
