3AI9
Crystal structure of DUF358 protein reveals a putative SPOUT-class rRNA methyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797, 0.9799, 0.95 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.288, 48.133, 62.362 |
| Unit cell angles | 90.00, 126.09, 90.00 |
Refinement procedure
| Resolution | 34.060 - 1.550 |
| R-factor | 0.19193 |
| Rwork | 0.190 |
| R-free | 0.23682 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 25283 | |
| <I/σ(I)> | 36.1 | 2.5 |
| Completeness [%] | 85.4 | 56.2 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG, Mg, Tris, Ammonium Fluoride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
