3AI5
Crystal structure of yeast enhanced green fluorescent protein-ubiquitin fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.515, 65.649, 70.225 |
| Unit cell angles | 90.00, 130.34, 90.00 |
Refinement procedure
| Resolution | 27.100 - 1.400 |
| R-factor | 0.18022 |
| Rwork | 0.179 |
| R-free | 0.20873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gfl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.884 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (10.2.35) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.058 | 0.545 |
| Number of reflections | 62484 | |
| <I/σ(I)> | 22.3 | 2.58 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1% Tryptone, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
