3AHY
Crystal structure of beta-glucosidase 2 from fungus Trichoderma reesei in complex with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.830, 103.511, 94.802 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 29.900 - 1.630 |
| R-factor | 0.16704 |
| Rwork | 0.164 |
| R-free | 0.21771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e3z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.088 | 0.503 |
| Number of reflections | 210766 | |
| <I/σ(I)> | 20.9 | 4.5 |
| Completeness [%] | 96.9 | 95.3 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris, 20-22%(w/v) PEG 3350, 0.23M MgCl2, 12.5mM sodium formate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
