3AHS

Crystal Structure of Ustilago sphaerogena Ribonuclease U2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-01-25
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.978
Spacegroup name	P 31 2 1
Unit cell lengths	98.237, 98.237, 87.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.320
R-factor	0.163
Rwork	0.162
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3agn
RMSD bond length	0.012
RMSD bond angle	1.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.081	0.587
Number of reflections	114464
<I/σ(I)>	68.5	4
Completeness [%]	100.0	99.9
Redundancy	15	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	0.84M sodium dihydrogenphosphate, 1.26M dipotassium hydrogenphosphate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K