3AH7
Crystal structure of the ISC-like [2Fe-2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 87.575, 87.575, 73.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.920 - 1.900 |
| R-factor | 0.18341 |
| Rwork | 0.182 |
| R-free | 0.21562 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7h |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.558 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.810 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.116 | 0.350 |
| Number of reflections | 13390 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.6 | 97.8 |
| Redundancy | 9.1 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6M trisodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
