3AEI

Crystal structure of the prefoldin beta2 subunit from Thermococcus strain KS-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-11-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 4 21 2
Unit cell lengths	67.315, 67.315, 92.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.660 - 1.700
R-factor	0.21
Rwork	0.210
R-free	0.24000
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Total number of observations	307371
Number of reflections	24056

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	500mM ammonium sulfate, 500mM ammonium chloride, 100mM sodium citrate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	500mM ammonium sulfate, 500mM ammonium chloride, 100mM sodium citrate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K