3AED

Crystal structure of porcine heart mitochondrial complex II bound with 2-Iodo-N-phenyl-benzamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	71.523, 84.074, 295.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.660 - 3.520
R-factor	0.226
Rwork	0.224
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zoy
RMSD bond length	0.006
RMSD bond angle	0.932
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.164	0.623
Number of reflections	22670
<I/σ(I)>	7.964	1.58
Completeness [%]	99.2	96.2
Redundancy	4.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	25mM HEPES-NAOH, 6% PEG 4000, 200mM Sucrose, 100mM NaCl, 10mM CaCl2, 0.5mM EDTA, 3% 1,6-haxanediol, 0.5% n-decyl-beta-D-maltoside, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K