3ADY
Crystal structure of DotD from Legionella
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97897 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.860, 103.860, 103.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.720 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.410 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 12739 | |
| <I/σ(I)> | 19.9 | 5.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | 8% PEG 8000, 0.1M CHES-NaOH, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
