3ACT
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.360, 98.315, 104.332 |
| Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.13077 |
| Rwork | 0.128 |
| R-free | 0.17630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cqt |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.839 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.092 | 0.384 |
| Number of reflections | 141447 | |
| <I/σ(I)> | 15 | 2.86 |
| Completeness [%] | 97.7 | 95 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | Na/K phosphate, VAPOR DIFFUSION, temperature 277K |
