3ACS
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase W488F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.796, 98.139, 104.376 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.14555 |
| Rwork | 0.142 |
| R-free | 0.21129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cqs |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.595 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.311 |
| Number of reflections | 57216 | |
| <I/σ(I)> | 14.2 | 2.89 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | ammonium sulfate, VAPOR DIFFUSION, temperature 277K |
