3ACC

Crystal structure of hypoxanthine-guanine phosphoribosyltransferase with GMP from Thermus thermophilus HB8

Replaces:  2YWT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-03-18
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	67.139, 67.139, 152.543
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.240 - 2.160
R-factor	0.21
Rwork	0.210
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3acb
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.160	2.160
Rmerge	0.086	0.163
Number of reflections	10796
Completeness [%]	93.4	86.1
Redundancy	10.2	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% Glycerol, 28% Dioxane, 0.005M GMP, 0.01M Magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K