3ABI

Crystal Structure of L-Lysine Dehydrogenase from Hyperthermophilic Archaeon Pyrococcus horikoshii

Replaces:  3A63Replaces:  2Z2V

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99321, 1.00900, 0.99284, 1.00
Spacegroup name	P 21 21 21
Unit cell lengths	75.291, 113.011, 122.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.510 - 2.440
Rwork	0.225
R-free	0.23550
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	83.045
High resolution limit [Å]	2.440
Number of reflections	39442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	2.1M AMMONIUM SULFATE, 0.1M CITRATE BUFFER, PH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K