3AB1
Crystal Structure of Ferredoxin NADP+ Oxidoreductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9831 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.514, 127.971, 128.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.570 - 2.390 |
| R-factor | 0.24232 |
| Rwork | 0.240 |
| R-free | 0.28891 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.400 |
| Rmerge | 0.080 | 0.485 |
| Number of reflections | 31262 | |
| <I/σ(I)> | 12 | 3.92 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 16 % PEG 6000, 0.1M ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 2 |
