3AAM
Crystal structure of endonuclease IV from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-07-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.646, 85.273, 36.580 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 29.120 - 1.580 |
| Rwork | 0.199 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xp3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
| Rmerge | 0.068 | 0.034 | 0.161 |
| Number of reflections | 29574 | ||
| <I/σ(I)> | 43.564 | ||
| Completeness [%] | 99.2 | 99.6 | 93.9 |
| Redundancy | 6.1 | 6.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 20mM MnCl2, 0.2M ammonium phosphate monobasic, 20% polyethylene glycol 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
