3AAM
Crystal structure of endonuclease IV from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-07-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.646, 85.273, 36.580 |
Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
Resolution | 29.120 - 1.580 |
Rwork | 0.199 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xp3 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.068 | 0.034 | 0.161 |
Number of reflections | 29574 | ||
<I/σ(I)> | 43.564 | ||
Completeness [%] | 99.2 | 99.6 | 93.9 |
Redundancy | 6.1 | 6.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 20mM MnCl2, 0.2M ammonium phosphate monobasic, 20% polyethylene glycol 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |