3A9L
Structure of Bacteriophage poly-gamma-glutamate hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.96400 |
| Spacegroup name | P 32 |
| Unit cell lengths | 86.809, 86.809, 85.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.040 - 1.900 |
| R-factor | 0.28291 |
| Rwork | 0.281 |
| R-free | 0.31495 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.040 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.410 |
| Number of reflections | 56281 | |
| <I/σ(I)> | 15.19 | 7.69 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 10.1 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40%(v/v) PEG 300, 5%(w/v) PEG 1000, 0.1M Tris buffer pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
