3A8R

The structure of the N-terminal regulatory domain of a plant NADPH oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2006-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	60.418, 72.162, 118.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.470 - 2.400
R-factor	0.235
Rwork	0.234
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0041)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.048	0.234
Number of reflections	20590
<I/σ(I)>	16.3	6
Completeness [%]	97.7	86
Redundancy	6.7	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K