3A73
Crystal Structure Analysis of Human serum albumin complexed with delta 12-prostaglandin J2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2008-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.060, 92.047, 94.664 |
| Unit cell angles | 74.80, 89.53, 80.21 |
Refinement procedure
| Resolution | 35.000 - 2.190 |
| R-factor | 0.23252 |
| Rwork | 0.229 |
| R-free | 0.29080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Rmerge | 0.072 | 0.113 |
| Number of reflections | 62497 | |
| <I/σ(I)> | 14.2 | 4496 |
| Completeness [%] | 92.0 | 91.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 50mM phosphate buffer pH 6.5, 28-32% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
