3A6V
Crystal structure of the MutT protein in MN(II) bound holo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 289 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-12-01 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.750, 55.950, 74.120 |
| Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a6s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.064 |
| Number of reflections | 19810 | |
| <I/σ(I)> | 28.6 | 18.6 |
| Completeness [%] | 98.1 | 96.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 1.4M POTASSIUM SODIUM TARTRATE, 87mM HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
