3A5V
Crystal structure of alpha-galactosidase I from Mortierella vinacea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0313 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 142.470, 142.470, 130.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.160 - 2.000 |
| R-factor | 0.1433 |
| Rwork | 0.142 |
| R-free | 0.17008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uas |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.291 |
| Data reduction software | DPS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.343 |
| Number of reflections | 45399 | |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M trisodium citrate dihydrate, 0.1M Tris buffer pH 8.5, 30% polyethylene glycol 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
