3A59
Structure of Hemoglobin from flightless bird (Struthio camelus)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 82.051, 82.051, 214.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 3.410 |
| R-factor | 0.2748 |
| Rwork | 0.265 |
| R-free | 0.35371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fs4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 3.540 |
| High resolution limit [Å] | 3.410 | 3.410 |
| Rmerge | 0.151 | |
| Total number of observations | 43272 | |
| Number of reflections | 13030 | |
| <I/σ(I)> | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 60% Poly ethylene glycol 3350, 50mM Sodium phosphate buffer pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
