3A3R
Structure of N59D HEN EGG-WHITE LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 281 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-12 |
| Detector | MAC Science DIP-320 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.240, 79.240, 38.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.20689 |
| Rwork | 0.202 |
| R-free | 0.24948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lza |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | PROTEIN |
| Data scaling software | PROTEIN |
| Phasing software | REFMAC (5.5.0088) |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.084 |
| Number of reflections | 8438 |
| Completeness [%] | 83.1 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 10% sodium chloride, 0.1M sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
