3A35
Crystal structure of LumP complexed with riboflavin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-28 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.288, 46.582, 161.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.150 - 1.421 |
R-factor | 0.19632 |
Rwork | 0.192 |
R-free | 0.23443 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ddy |
RMSD bond length | 0.033 |
RMSD bond angle | 2.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.559 |
High resolution limit [Å] | 1.421 |
Number of reflections | 58164 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20% (w/v) PEG 4000, 0.2M MgCl2, 100mM HEPES pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |