3A0E
Crystal Structure of Polygonatum cyrtonema lectin (PCL) complexed with dimannoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-10 |
Detector | MAR555 FLAT PANEL |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.660, 76.660, 61.401 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.080 - 2.000 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a0d |
RMSD bond length | 0.005 |
RMSD bond angle | 1.470 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.080 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.076 | 0.265 |
Number of reflections | 14437 | |
<I/σ(I)> | 8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0M LiSO4, 2% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |