3A02

Crystal structure of Aristaless homeodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 32 2 1
Unit cell lengths	45.810, 45.810, 48.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.230 - 1.000
R-factor	0.17279
Rwork	0.172
R-free	0.18436
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fjl
RMSD bond length	0.024
RMSD bond angle	1.943
Data scaling software	XSCALE
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.670		1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.083	0.066	0.450
Rmeas	0.087	0.070	0.480
Total number of observations	327246
Number of reflections	32230	261	2340
<I/σ(I)>	18.23	26.2	5.8
Completeness [%]	99.0	62.3	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	30% PEG 400, 0.1M Cadmium chloride, 0.1M Acetate, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K