39PB

Crystal structure of a septum formation protein Maf from Coccidioides immitis (AMP complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-09-26
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 43 21 2
Unit cell lengths	76.223, 76.223, 174.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.300 - 2.330
R-factor	0.2113
Rwork	0.209
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.2.1_6174: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	2.410
High resolution limit [Å]	2.330	2.330
Rmerge	0.086	1.987
Rmeas	0.088	2.028
Rpim	0.018	0.402
Total number of observations	587279	55108
Number of reflections	22907	2195
<I/σ(I)>	24.4	1.8
Completeness [%]	100.0
Redundancy	25.6	25.1
CC(1/2)	0.999	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Morpheus Fusion G7: 12.5% (v/v) MPD; 12.5% PEG 1000; 12.5% (w/v) PEG 3350; 0.05M Tris (base); 0.05M BICINE pH 8.5; 0.3% w/v NDSB 195, 0.3% w/v NDSB 201, 0.3% w/v NDSB 211, 0.3% w/v NDSB 221, 0.3% w/v NDSB 256; 0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide , CoimA.00961.a.A2.PW39528 at 13.2 mg/mL. cocrystallization with 25 mM AMP disodium salt and 20 mM Magnesium chloride hexahydrate. plate 21398 G7 drop 1, Puck: PSL-0109, Cryo: direct.