39JP
Crystal structure of aminopeptidase P from Lactococcus lactis in complex with phosphate at 1.83 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-06-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 54.410, 79.590, 88.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 1.830 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.083 | 2.013 |
| Number of reflections | 34244 | 2371 |
| <I/σ(I)> | 15.6 | 0.9 |
| Completeness [%] | 98.6 | 89.2 |
| Redundancy | 12.2 | 7.1 |
| CC(1/2) | 0.999 | 0.342 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.1 M Tris pH 8.5, 2.0 M ammonium dihydrogen phosphate |
