399D
STRUCTURE OF D(CGCCCGCGGGCG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.290, 42.602, 45.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.211 * |
| Rwork | 0.165 |
| R-free | 0.16800 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 17.103 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.180 |
| Number of reflections | 4195 * | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 87.5 | 79.7 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | pH 5.60, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PROTEIN (PHI0) | ||
| 3 | 1 | 1 | NA CITRATE | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | AMMONIUM ACETATE | ||
| 6 | 1 | 1 | PEG 4000 | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | duplex | 0.7 (mM) | |
| 2 | 1 | drop | protein | 0.5 (mM) | |
| 3 | 1 | drop | sodium chloride | 0.2 (M) | |
| 4 | 1 | drop | ammonium acetate | 0.15 (mM) | |
| 5 | 1 | drop | sodium citrate | 0.05 (M) | |
| 6 | 1 | drop | PEG4000 | 16 (%(v/v)) | |
| 7 | 1 | reservoir | ammonium acetate | 0.7 (M) | |
| 8 | 1 | reservoir | PEG4000 | 25 (%(v/v)) |
