38WT
Crystal structure of the CFTR inhibitory factor Cif with the L174A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92019 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.707, 84.197, 89.526 |
| Unit cell angles | 90.00, 100.34, 90.00 |
Refinement procedure
| Resolution | 43.730 - 1.520 |
| R-factor | 0.1576 |
| Rwork | 0.157 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.730 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.051 | 0.614 |
| Rmeas | 0.058 | 0.717 |
| Rpim | 0.027 | 0.361 |
| Number of reflections | 186606 | 17976 |
| <I/σ(I)> | 14.01 | 1.77 |
| Completeness [%] | 98.7 | 95.57 |
| Redundancy | 4.4 | 3.8 |
| CC(1/2) | 0.999 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 15% (w/v) PEG 8000, 200 mM calcium chloride, 100 mM sodium acetate, pH 5.0 |
