38MS

[DXL-6|2] Double crossover-like motif with mobile semi-junctions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920208
Spacegroup name	P 43
Unit cell lengths	34.789, 34.789, 102.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.810 - 1.680
R-factor	0.249
Rwork	0.246
R-free	0.30240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.272
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.810	28.807	2.257
High resolution limit [Å]	1.680	6.716	1.683
Rmerge	0.094
Number of reflections	4434	222	222
<I/σ(I)>	8.6
Completeness [%]	85.9
Redundancy	8.7
CC(1/2)	0.999	0.999	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate