38DC

X-ray Structure of the FAB Fragment of a GluA2-specific Murine Monoclonal Antibody 15F1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	62.461, 64.277, 204.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 2.500
R-factor	0.2112
Rwork	0.209
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.763
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.810	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.061	0.468
Rmeas	0.072	0.552
Rpim		0.290
Number of reflections	29417	3247
<I/σ(I)>	22	3.5
Completeness [%]	99.9	100
Redundancy	6.6	6.8
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Fab (11.6 mg/mL) was mixed at a 1:1 ratio with precipitant containing 0.1 M Tris (pH 7.0), 24% PEG 3350, and 20% ethylene glycol. Crystals were harvested and directly vitrified in liquid nitrogen without further cryopreservation