37FX

OYE1-His6 loop 6 variant - P295G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920174
Spacegroup name	P 43 21 2
Unit cell lengths	137.846, 137.846, 40.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 2.900
R-factor	0.3156
Rwork	0.312
R-free	0.35240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	Aimless (0.7.9)
Phasing software	PHENIX (1.20.1)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.720	3.010
High resolution limit [Å]	2.900	2.900
Number of reflections	9033	752
<I/σ(I)>	5.45
Completeness [%]	90.0
Redundancy	15.8
CC(1/2)	0.997	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	277	20 mg/mL protein in 25 mM Tris hydorchloride, 25 mM NaCl, 20% PEG 400, 0.1 M MgCl2, 50 mM HEPES