37CD

Crystal structure of GABARAP in complex with the LIR of MARV nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96860
Spacegroup name	P 21 21 21
Unit cell lengths	35.750, 70.063, 102.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.770 - 2.780
R-factor	0.2447
Rwork	0.240
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.770	3.060
High resolution limit [Å]	2.780	2.780
Number of reflections	11445	2116
<I/σ(I)>	4.44
Completeness [%]	86.2	62.17
Redundancy	2.9
CC(1/2)	0.982	0.083

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.02 M Sodium/potassium phosphate 0.1 M Bis-Tris propane 7.5 20 % w/v PEG 3350