37ON
Crystal structure of the Mucin-type O-glycosyltransferase PGANT5A-4M (A430T, L434N, A437N, S456Y)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-13 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.113, 89.170, 129.525 |
| Unit cell angles | 81.70, 81.73, 71.06 |
Refinement procedure
| Resolution | 29.770 - 3.080 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.518 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.770 | 3.150 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.120 | 0.965 |
| Rmeas | 0.170 | 1.365 |
| Rpim | 0.120 | 0.965 |
| Number of reflections | 44420 | 4339 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 94.7 | 96.7 |
| Redundancy | 1 | 1.7 |
| CC(1/2) | 0.962 | 0.224 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293.15 | PEG 3350, HEPES, magnesium chloride |
