378D
STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.550, 42.180, 43.380 |
Unit cell angles | 90.00, 96.56, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | COORDINATES OF D(CCCCCIIIII) (FROM CRYSTAL STRUCTURE) |
RMSD bond length | 0.004 * |
RMSD bond angle | 1.090 * |
Data reduction software | XENGEN (V. 2.1) |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.700 | 2.510 |
High resolution limit [Å] | 2.230 | 2.400 |
Rmerge | 0.070 | |
Number of reflections | 3588 * | |
Completeness [%] | 84.7 * | 53.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 293 * | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | |
2 | 1 | drop | distamycin A hydrochloride | 1 (mM) | |
3 | 1 | drop | magnesium chloride | 20 (mM) | |
4 | 1 | reservoir | MPD | 40 (%) |