376D
A ZIPPER-LIKE DNA DUPLEX D(GCGAAAGCT)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW21B |
| Synchrotron site | LURE |
| Beamline | DW21B |
| Temperature [K] | 276 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 37.560, 37.560, 65.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24100 |
| Structure solution method | MAD AT BROMINE EDGE |
| Starting model (for MR) | MANUALLY BUILT IN THE EXPERIMENTAL DENSITY |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.023 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MADLSQ |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.148 |
| Total number of observations | 21345 * | |
| Number of reflections | 1832 | |
| <I/σ(I)> | 11.3 | 4.2 |
| Completeness [%] | 95.0 | 61 |
| Redundancy | 11.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | CACODYLATE | ||
| 3 | 1 | 1 | [CO(NH3)6]3+ | ||
| 4 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1mg | |
| 2 | 1 | drop | lithium cacodylate | 0.05 (M) | 0.4ml |
| 3 | 1 | drop | 0.1 (M) | 0.003ml | |
| 4 | 1 | drop | cobalt hexammine chloride | 10 (mM) | 0.015ml |
| 5 | 1 | drop | MPD | 50 (%) | 0.008ml |
| 6 | 1 | reservoir | MPD | 25 (%) |
