36WJ

Mevalonate kinase from Saccharomyces cerevisiae with isopentenyl pyrophosphate (IPP) bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911
Spacegroup name	P 43 21 2
Unit cell lengths	83.450, 83.450, 262.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.540 - 2.070
R-factor	0.1977
Rwork	0.196
R-free	0.22470
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.970	2.140
High resolution limit [Å]	2.070	2.070
Number of reflections	56693	4331
<I/σ(I)>	5.6
Completeness [%]	98.9	99.1
Redundancy	4.5	4.7
CC(1/2)	0.958	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM MES pH 6.0, 900-1100 mM sodium potassium tartrate