36WI

Mevalonate kinase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	83.450, 83.450, 262.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.540 - 2.150
R-factor	0.1995
Rwork	0.198
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.450	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	49876	738
<I/σ(I)>	5.9
Completeness [%]	97.5	99
Redundancy	3.9
CC(1/2)	0.995	0.305

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM MES pH 6.0, 900-1100 mM sodium potassium tartrate