36MB
Crystal Structure of mini-binder MB07-ds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.306, 51.306, 56.008 |
| Unit cell angles | 90.00, 90.95, 90.00 |
Refinement procedure
| Resolution | 56.000 - 2.000 |
| R-factor | 0.2305 |
| Rwork | 0.227 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.538 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.240 | 1.361 |
| Rpim | 0.097 | 0.585 |
| Number of reflections | 26405 | 1891 |
| <I/σ(I)> | 5.23 | 0.91 |
| Completeness [%] | 99.1 | 99.41 |
| Redundancy | 6.8 | 6.2 |
| CC(1/2) | 0.985 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M Citric acid pH 3.5, 2.0 M Ammonium sulfate |
