36LL

16mer self-complementary RNA duplex with L-U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	H 3 2
Unit cell lengths	42.091, 42.091, 130.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.270 - 1.350
R-factor	0.2409
Rwork	0.239
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.074
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.270	1.440
High resolution limit [Å]	1.350	1.350
Number of reflections	8765	438
<I/σ(I)>	10.3
Completeness [%]	89.3
Redundancy	6.3
CC(1/2)	0.994	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.04 M Lithium chloride, 0.08 M Strontium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 7.0, 30%v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride