36LE
RNA-dGMP complex with L-G-terminal primer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91165 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 46.300, 47.423, 84.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.300 - 1.730 |
| R-factor | 0.2273 |
| Rwork | 0.224 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.513 |
| Data reduction software | autoPROC (1.0.5) |
| Data scaling software | autoPROC (1.0.5) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 1.920 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 14792 | 740 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 91.6 | 49.8 |
| Redundancy | 4.9 | |
| CC(1/2) | 0.996 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8,000 |
