36JA
Coagulation factor V DTQQ (B-domain region 811-1491 truncated, R709Q, R1545Q) in solution-phase
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Software | cryoSPARC,RELION,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
