36IW
Crystal structure of a large ribosomal subunit protein bL17 from Bordetella pertussis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-05-30 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.194, 73.010, 52.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.990 - 1.650 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_6082: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.990 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 1.453 |
| Rmeas | 0.063 | 1.519 |
| Rpim | 0.017 | 0.435 |
| Total number of observations | 165508 | 7074 |
| Number of reflections | 12571 | 595 |
| <I/σ(I)> | 20 | 1.8 |
| Completeness [%] | 99.5 | |
| Redundancy | 13.2 | 11.9 |
| CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 3M NaCl, 100mM Bis-Tris pH 5.5, BopeA.17872.a.B2.PW39472 at 11.5 mg/mL. Plate 20708 G12 drop 1, Puck: PSL-0509, Cryo: 20% glycerol + 80% crystallant |
