36AC
Ternary complex between CHD4-C1bC2ab, GATAD2ACR2, and CDK2AP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2025-11-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.732, 52.858, 72.473 |
| Unit cell angles | 98.75, 103.40, 110.69 |
Refinement procedure
| Resolution | 30.030 - 2.000 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5885) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.030 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | |
| Number of reflections | 36223 | 1812 |
| <I/σ(I)> | 22.02 | 1.8 |
| Completeness [%] | 94.3 | 89.95 |
| Redundancy | 2.6 | 2.2 |
| CC(1/2) | 0.997 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M sodium malonate, pH 7.0, and 20% (w/v) PEG 3350 |
